YvesLeb I was able to focus the Moon with a 25mm eyepiece and had a very clear image in focus.
Are you certain that it is a focus issue with the Moon, and not an exposure issue?
Take a look at the Max ADU number just to the right of the guide graph in the main window, Reduce the exposure time and the gain of the camera so that the Max ADU is under 65000.
If that still produces a bloated moon, then you need to adjust focus. While you are attempting focusing, monitor the Max ADU to make sure you are not overexposed.
It is even easier if you have a clear, moonless night. Point the OTA at a bright star. If you can see a dim but very large disk (even the size of your sensor), you are halfway there. Adjust the focus in one direction and then the other direction, and observe the Max ADU again. As you approach focus, Max ADU will increase (the total flux from the star lands on fewer pixels of the camera sensor). At the same time, keep re-adjusting the exposure and gain to stay below 65000. As long as the defocused star's flux is above the camera noise, this method will work even if you cannot visually see a large disk at the beginning. Just rack the focuser in and out to see which direction gives you a larger Max ADU.
By adjusting for largest Max ADU, it should get you a compact star that is close enough to use a Bahtinov Mask to finely focus the telescope.
If you hit the focuser limit, you will need to shorten or lengthen the back-focus distance.
If you are not using any focal reducer with your 5SE, the back focus should be close to 127mm. This is the distance from the metal back of the SCT to the sensor in your camera. 127mm (5 inches) may seem quite long, but your telescope is meant for visual viewing usng a star diagonal; the star diagonal needs a large back-focus distance.
If you have the large hockey puck shaped ASI290, its flange to sensor distance is 12.5mm, if you are using the cooled ASI290, the flange to sensor distance is 17.5mm. Subtract these numbers from the 127mm back-focus value to get the distance that the flange of your ASI290 needs to be placed behind the metal back of your telescope.
If you are using a Celestron 0.63x flattener/reducer, the back-focus from the metal back of the reducer to your camera sensor should be 105mm.
Chen