I have also seen this problem and have sent a bug report to ZWO support.
I am using a recently purchased a ZWO ASI294MM Pro camera with the unlocked bin x1 mode.
I am using the camera with MaximDL v6.21 and FocusMax v4.1.0.102 for imaging. I have the ASI Native Camera driver v3.0.0.11 and ASCOM driver v6.5.1 installed. I am controlling the camera using MaximDL through ASCOM.
Calculation of sub-frame dimensions and coordinates in bin x1 is correct.
However, when I image and focus the camera in bin x2 I get sub-frame errors. There is an error in calculating the bin x2 sub-frame dimensions and coordinates with the ASCOM driver.
The ASCOM driver fails to locate the sub-frame window within the image and read the sensor correctly which causes a series of problems focussing and imaging with the camera at bin x2.