FlyData I am using the ASIImg program in the ASI suite to operate my camera.
May I suggest a couple of controlled tests?
First, set the camera up indoors. Connect the 12V supply, but turn cooling off. Completely static (nothing moves - camera does not move, none of the connectors moves), and set up an autorun. Does it work or fail? If it fails, you might have a defective camera. But check to make sure the 12V connector is a high quality 5.5mm/2.1mm one, not a 5.5mm/2.5mm one. Another thing to try is a dumb USB hub in between the computer and the camera -- do not use a hub that has USB-PD (power delivery) support. If you have an oscilloscope, check for noise from the power supply -- even battery packs can have noisy buck/boost converters in the line -- so "batteries" can be be noisy too, unless you are going directly from a 12V SLA battery that is not connected to anything else.
If the above works, take the same setup outdoors in the cold. Keep the cooler off, but again keep the 12V supply on. Again, nothing is allowed to move. Use exactly the same cables and wiring that you used indoors. Does it work or does it fail? If it fails, you have a temperature dependency somewhere - power supply, connectors, the camera electronics...
If the above works, bring up the cooler to normal stress (but never more than 90%). Does it work or does it fail? If it has worked until now, but fails when you start cooling the camera, I would check the loaded voltage of the power supply, and the voltage drop in the power cable. It could be other things too, like vibration of the fan shaking the connectors.
If it still works, start the mount tracking (no need to autoguide). Does it still work? If not, your connectors could be loose.
Chen