Hi... I am new to this forum, so please excuse any duplicate questions. I am trying to use the ASI183MM PRO in a microscopy project and to control the camera using opencv and EMGU (the VB.net wrapper of opencv). Everything works great for camera control and capture of an 8 bit image. However, I would like to run the camera at high bit depth and cannot seem to switch the camera to 12 bit mode and 16 bit readout. There appears to be a FOURCC get command which returns the value 466162819 (BTW, what does this mean?), but using this command to set a value does not seem to have any impact. Any suggestions on whether or not this mode can be accessed through opencv or EMGU?