Set global gain to Unity (120) in ASIAIR version 2.1.1(10.74) Generate a frame sequence using Autorun to say, 30 repetitions The first 16 will have a gain of 120 while the rest will have a gain of 100 This was confirmed by examining FITS headers in PixInsight
I tested, but did not reproduce the problem. Gobal gain value is read from the gain in the main camera settings. Did you change this during autorun?