The ASI533MC Pro is not a DSLR camera. It is a dedicated CMOS astronomical camera. These color cameras create images in what is known as a Bayer pattern. Any program you use to open or process them needs to "debayer" the image to get rid of the "pixelation". What were you using to do this? To eliminate noise you need to create "darks" which when stacked with the light images will eliminate a lot of the noise.
There are numerous programs you can use to take the images.
- For Deep Space objects you can use:
a. ZWO's ASIStudio's ASIImg and stack with ASIDeepStack
b. SharpCap
c. NINA
to name a few.
- For Lunar, Planetary, and Solar (normally you would capture videos for this,preferable in .SER format)
a. ZWO's ASIStudio's ASICap and stack with ASIVideoStack
b. SharpCap
there may be others.
For Processing images, there are numerous options out there. Some include stacking and some do not.
- PixInsight is the most popular but has a rather steep learning curve and a little expensive.
- Photoshop (again rather expensive)
- Affinity Photo is good, does not cost you an arm and a leg)
- Siril is very good and "free".
There are numerous others.
Regarding backfocus that depends on your telescope and whether you are using a focal reducer. Go out in the daytime and focus at a long distance object to determine a good approximation. That may need to be tweaked a bit when working on an actual sky object.
The ASI533MC Pro is a great camera. I have gotten good results with it.
Good luck,
JohnD