Thanks for the quick response.
When I used Registax 6 I could only load one dark, no bias. I did try to load a master dark the result was similar.
I regularly see people using small number of darks with good results, so I limited the number of darks to save time.
In the DSS image, I could load all my darks and biases. The stacked image did show thermal noises successfully removed, just not sure what are the diagonal line noise. I doubt biases will help because I don't see any diagonal lines in biases, or in darks/lights. Where did they come from?
BTW, during my very first astrophoto session I also did the Dumbell (image posted here before) with 100 lights and 20 darks, no biases, after DSS there was no diagonal lines. Don't know what has changed.