Hi Sam,
I spent over 7 hours battling last night and the result is pretty poor, I am not sure what happened here as I have been doing a lot of research and thought I had it right. here is the TIFF file of Messier 78 :
1- 54 Exposures 3 minutes long
2- Gain was 240 and Offset 65
3- The night temperature was 27 degrees centigrade and the camera was -15c
4- 70 Flat frames with Median ADU of 21,000
5- No Darks
I have lots of vertical noise !!!!
Lots of Amp glow
The Colors are also not right too
Processed with Deep sky Stacker with the Color bayer matrix set as GRBG
Any idea what went wrong here as I am quite distressed.
Your help is well appreciated
Here is the link to the stacked image
https://www.dropbox.com/s/49jwvay2r7m84mj/Messier-78-ZWO.TIF?dl=0
I am quite certain there is something wrong with the settings but after reading so many articles I thought I had it right but the image is shocking, your assistance is well appreciated.