Hi Sam and Sean,
I am experiencing a similar colored background in the integrations of my ASI294MC Pro using a Takahashi FSQ 106N. This artifact is neither caused by light pollution nor is it IFN signal: I integrated 50 frames before meridian flip and 60 frames thereafter (no rotator was used). The problematic background was at the same sensor positions, which is evident in the appended images ("Image_1.JPG" and "Image_2.JPG", STF Autostretch applied in PixInisght).




Acquisition conditions: image acquisition (light frames as well as calibation frames ) with Sequence Generator Pro, exposure time 50 and 60 x 150 s respectively at gain 120, -15 °C, dithering applied. I did not use high cooling power: the set temperature was -15 °C at an ambient temperature of 14 - 12 °C, so the temperature difference was actually only 29 - 27 degrees. The cooling power was always below 50 %. I guess that the settings for the cooler have been well suited, and I don't like to go up with temperature because of thermal noise.
It's intruiging to me that this artifact is well reproducible: the light frames for Image_3.JPG (190 x 150 s at gain 120, -15 °C, dithering applied) were taken 1 month before. Compared with the 2 images above the pattern of the colored background artifact again appears at the same regions of the sensor.


My workflow (in PixInsight): Image calibration with MasterDark and MasterFlat , Debayer, Alignment, Integration).
The artifact definitely is not present in my MasterDark. The debayered MasterDark (same stretching applied as in the integration) shows 'amp glow', but not a trace of colored background is detectable in it. Furthermore, when I subtract the bias-offset from each light frame and then calibrate with MasterFlat, the same colored background with the same strength results after debayering, alignment and integration. So this artifact is only in the light frames.
I suspected that my MasterFlat, that was based on flat frames with low exposure time, was the culprit. So I made new flat frames with an exposure time of 3 s. Using this new MasterFlat for Image calibration, the resulting new integration was not distinguishable from the former one.
I transferred a zip archive of images to a filehoster and gave the link to ZWO, but unfortunately ZWO has not managed to download them due to file size.
At this point I don't know what to do next. The light frames of this camera do not seem to calibrate properly. I need your support and hope you are willing to help.
My ASI294MC Pro had the following Firmware versions:
FW version: V 5.0
FPGA FW version: 294 V 1.0.2.7
Bernd