Hi there
It could be the offset problem.
In sharpcap, for deepsky, following parameter must be the same for all frames.
color balance: R=50, B=50
Gain= same for all frames.
offset(brightness)= same for all frames(value>50)
exposure= dark and light should be same
color space=RAW16
Format= *.fit
I highly recommend to use some professional deepsky capture softwares. Such as SGP, Maxim DL, The SkyX, ATP, etc.
That will help you get better result, they design for DSO imaging.