Okay.
I didn't remove the 1600 from my OAG setup, so I took the following precautions; I had a black 'filter' (black aluminium foil) in place in the filter wheel, and the guide camera in place (no light coming in through the stalk) and aluminium foil in place over the rubber lens cap of the Field Flattener.
I wanted to work with exact ADU levels as near as I could determine experimentally using Stark's method, so the data would be in multiples of each other eg;
76 = 2
100 = 1.5
135 = 1
195 - 0.5
253 = 0.25
313 = 0.125
For each gain setting I shot 250 bias frames at 0.000032s each, integrated them with the settings below in Pixinsight. Then I used statistics to read out the mean value from the integrated master bias.
I then took 2 or 4 dark frames (depending on which run of tests it was - I did a second run to verify some data points) with exposures at 30s, 60s, 120s, 300s and 600s in SGP. Temperature was -15C for all subs (and bias). The setup for the dark frames was the same as for the bias.
In statistics, I took the mean values and averaged them in a spreadsheet.
The result of shooting those gain settings at various exposure lengths is what you see in the post above.
Best,
Markus