@matt s#55378
"The issue with the IMX294 sensor is that amp glow becomes prominent at exposures below 1 second. A one second exposure has about the same amp glow as a 20 second exposure. Amp glow drops sharply at 1 second and then shows normal growth above the one second mark. For exposures of zero to one second, amp glow ramps up rapidly." - by jdupton, 02 April 2021 CloudyNights
ps. I also should mention that you should combine the longer exposure flats with darks and flat darks, no biases.