Kevin_A using HGC increased the hot pixels substantially only so I do not find that would be good overall.
Back of the envelope... Dark current noise at -10ºC is about .002e-/sec, or 0.36e- with 180 sec exposure. At Unity gain, this (0.36e-) should be the actual (unity) contribution of random sensor noise, while the read noise is at a whopping 3.8e-.
With HCG, the RMS random noise is increased by a factor of 2 (HCG kicks in at a gain of about 5.4 dB above Unity Gain), so the 0.36e- dark current noise becomes 0.72e-, while read noise falls to about 1e- : the total noise (RMS) is now about 1.25e-. This should be better than the 3.8e- at Unity gain.
Perhaps I am missing something. If not, are we seeing mostly read noise at Unity Gain and 180 second exposure at -10ºC?
Also, if dark current is the problem, then -20ºC should help. But if read noise is the problem, that extra 10ºC cooling will do nothing.
Chen